Bacterial Growth Inhibitory Effect of Ceratonia siliqua L. Plant Extracts Alone and in Combination with Some Antimicrobial Agents Pages 3-13

Alyaa Hatem Ibrahim1,Rehab Mahmoud Abd El-Baky2,Samar Yehia Desoukey1, Ahmed Abd-Lateff1,3 and Mohamed Salah Kamel1

1Pharmacognosy Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 2Microbiology Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 3Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, PO Box 80260, Saudi Arabia

DOI: http://dx.doi.org/10.12970/2311-1755.2013.01.01.1

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Abstract: Objectives: To evaluate the antibacterial activity of the total extracts, methanolic and water extract of leaves with stems and the pods of Ceratonia siliqua L. each alone and in combination with some antimicrobials on some pathogens. As the wide use of antibiotics in the treatment of bacterial infections has led to the emergence and spread of resistant strains. Many studies showed that the efficacy of antimicrobials can be improved by combining them with crude plant extracts.

Materials and Methods: the antibacterial activity of the total extracts, methanolic and water extract of leaves with stems and the pods of Ceratonia siliqua L. each alone and in combination with some antimicrobials was evaluated using well-diffusion method. Cytotoxicity of the total methanolic extract against Huh-7 liver and A-495 lung cancer cell lines was assessed using SRB method.

Results: Well diffusion method demonstrates an in-vitro antibacterial activity of the tested extracts against tested microorganisms. Combination of the tested extracts with antimicrobials increased the activity of the tested antimicrobials. A dose dependant effect on both Huh-7 liver and A-495 lung cancer cells was observed.

Conclusion: Our results revealed the importance of plant extracts when associated with antibiotics to control resistant bacteria that become a threat to human health. In addition, the tested plant extracts can be exposed for further investigation to be used as hepatoprotective agent.

Keywords: Ceratonia siliqua L plant extract, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, combination, antimicrobial agents. Read more