Molecular Diagnosis and Seroepidemiology of Bovine Viral Diarrhoea Virus in Cattle with Abortion Problem
DOI:
https://doi.org/10.12970/2310-0796.2015.03.02Keywords:
Abortion, BVDV, Cattle, ELISA, RT-PCR.Abstract
This study is a serological and virological investigation of the bovine viral diarrhoea virus (BVDV) infection in cattle with abortion problem from private small scale production units of less than 20 cattle per unit, in the Kars province of Turkey. For this purpose, blood sera from 188 cattle were tested for antibodies against BVDV using a commercial enzyme-linked immunosorbent assay (ELISA). Since the virus causes persistent infection, antigen-ELISA was also performed for this agent. Seropositivity rate was detected to be 60.63%. In addition, the BVDV antigen was not detected in seronegative sera samples. Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of BVDV nucleic acid. BVDV nucleic acid was found in 2 of 74 seronegative samples (2.7%) by RT-PCR. The results suggest that the infection was spreading in private small scale production units. Furthermore, recommendations for the control of BVDV infection are presented.
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