Identification of miRNA-mRNA Interaction in Vero Cells after Infection of SADS-CoV through Integrative Transcriptome Analysis

Authors

  • Yaoyao Zheng College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Jiaoling Wu College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Yuqi Chen College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Xiaoyu Tang College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Yaorong Tan College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Yuan Sun College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Qianniu Li College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Ruiting Wu College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Xiangbin Zhang College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China
  • Jingyun Ma College of Animal Science, South China Agricultural University, Guangzhou, China and Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China

DOI:

https://doi.org/10.12970/2310-0796.2020.08.06

Keywords:

 SADS-CoV, Vero cells, Differentially expressed miRNA, Differentially expressed mRNA, miRNA-mRNA-pathway network.

Abstract

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus that can cause severe diarrhea in newborn piglets in China. Here, we reported profiles of differentially expressed miRNAs and mRNAs in Vero cells infected by SADS-CoV. In total, 345 miRNAs and 1014 mRNAs were identified with significantly differential expression after SADS-CoV infection. The analysis of miRNA-mRNA-pathway network indicated that through major mRNA nodes, TUBA, KRAB, eIF4E and SIRPA_B1_G, differentially expressed miRNAs were mainly involved in pathways of pathogenic Escherichia coli infection, apoptosis, PI3K-Akt signaling pathway, focal adhesion and so on. The qRT-PCR validation results of six selected genes were concordant with RNA-Seq results. This study is the first report to examine differentially expressed miRNAs and mRNAs after SADS-CoV infection in Vero cells, which may help elucidate the underlying mechanisms of SADS-CoV infection. 

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Published

2020-04-20

Issue

Vol. 8 (2020)

Section

Articles