The Effect of Platelet Rich Plasma on The Survival of Follicles in Hair Transplantation: An Experimental Study on Rats
Authors
Cetin Duygu1, Ethem Guneren2, Nur Buyukpinarbasili2 and Oznur Inan3 1Malatya State Hospital, Malatya, Turkey; 2Bezmi Alem Vakif University, Istanbul, Turkey; 3Istanbul Mehmet Akif Ersoy Education and Research Hospital, Turkey
Background: Platelet Rich Plasma (PRP) is currently used for a wide variety of medical applications and involves IGF, VEGF, EGF which are important in angiogenesis and endothelial differentiations. EGF, IGF, TGF- β, are other substance of PRP and simultaneously effect to hair growth. Objectives: The authors decided to observe the influence of PRP to the success rate and vascularization rate of hair transplantation. Methods: Control and PRP application sides are determined on the dorsal skin of rats. 2x1 cm skin islands were excised and punched to get out hair follicle grafts. Then hair follicles were transplanted into subcutaneous dorsal pockets. Injection of 0.2 ml PRP was made in to the pouch. 1 (20 rats) and 2 (20 rats) months after transplantation, skin sample including the hair follicle were removed and analyzed by immunochemical CD31 and direct microscope. Results: After 1 month, control and PRP groups exhibited similar density of follicle shaft. But degree of capillary formation in PRP group was higher than that of the control groups. After 2 months, degree of capillary formation and follicle shaft numbers were significantly higher than that of control groups. Conclusions: Increase of capillaries were correlated PRP involving IGF, VEGF, EGF which are important in angiogenesis and endothelial differentiations. Increase of hair shaft number was correlated with PDGF’s mitogen and chemoattractant effect to mesenchymal cells and stem cells. Also PRP involves EGF, IGF, TGF- β, these affect hair growth. In conclusion, PRP and hair transplantation collocation to may enhance the success of hair transplantations.
