New Tool for Screening of Whole Blood for Early Detection of Breast Cancer Antigen (CA153)
DOI:
https://doi.org/10.12970/2308-8044.2013.01.02.3Keywords:
Breast cancer antigen, screening, stochastic microsensor.Abstract
Nanostructured materials play an important role in the design of new tools for medical use. Therefore materials such as maltodextrins were excellent candidates for the design of stochastic microsensors used in the screening of whole blood for breast cancer biomarker. The results obtained for the assay of breast cancer antigen in whole blood using this microsensor (by applying a potential of 125mV vs Ag/AgCl and performing measurements specific to stochastic sensing described in detail in the article) were in agreement with those obtained using the standard method, ELISA (Enzyme-Linked Immunosorbent Assay utilized a monoclonal anti-CA 15-3 antibody directed against intact breast cancer antigen, CA 153 for solid phase immobilization - on the microtiter wells. The concentration of CA 153 was directly proportional to the color intensity of the test sample.) for serum samples. The limit of determination obtained for breast cancer antigen (0.5mU/mL) when assayed in whole blood, proved that the microsensor is a good tool (in terms of sensitivity, selectivity, and fast response) for early screening of whole blood for breast cancer.
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