Comparative Analysis of In Vitro Antibacterial Effectiveness of Ozonized and Non-Ozonized Vegetable Oils on Neisseria gonorrhoeae

Authors

  • Morisa Martins Leão Carvalho Medical School, Universidade Brasil, Fernandópolis, Brazil
  • Dora Inés Kozusny-Andreani Medical School, Universidade Brasil, Fernandópolis, Brazil
  • Fernando Batigália Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil
  • Ademir Barianni Rodero Medical School, Universidade Brasil, Fernandópolis, Brazil
  • Nilton Cesar Pezati Boer Medical School, Universidade Brasil, Fernandópolis, Brazil
  • Renato Amaro Zângaro Biomedical Engineering Institute - Anhembi Morumbi University-São José dos Campos, Brazil and Center for Innovation, Technology and Education-CITÉ, São José dos Campos, Brazil
  • Rogério Rodrigo Ramos Medical School, Universidade Brasil, Fernandópolis, Brazil

DOI:

https://doi.org/10.12970/2308-8044.2020.08.05

Keywords:

 Neisseria gonorrhoeae, Bactericidal agents, Ozone, Oxigen-reactive species, Alternative treatment.

Abstract

 The bacterium Neisseria gonorrhoeae, responsible for bacterial infection known as gonorrhea, have become a public health problem because of its increasing therapeutic resistance. To evaluate the antibacterial effectiveness of ozonized vegetable oils and non-ozonized oils (sunflower, coconut, palm and olive) on Neisseria gonorrhoeae. The standard strain of Neisseria gonorrhoeae ATCC 49226 was evaluated against ozonized and non-ozonized sunflower, coconut, palm and olive oils. The antibacterial activity of the oils, in concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.562% and 0.781, was obtained by disk diffusion and broth microdilution. Positive controls comprised ceftriaxone and ciprofloxacin, and negative controls, dimethylsulfoxide and Tween 20. The minimum inhibitory concentration and minimum bactericidal concentration were evaluated. The experimental design was completely randomized. Ozonized sunflower oil showed greater bactericidal action, followed by olive and palm oils. Multivariate clustering approach made it possible to confirm that sunflower oil was more effective against Neisseria gonorrhoeae, followed by palm and olive oils, and the coconut oil was the least efficient. In the comparative analysis of the antibacterial effectiveness of ozonized vegetable oils on Neisseria gonorrhoeae, although the palm and olive oils present antibacterial activity, the ozonized sunflower oil showed high efficiency in reduced minimum inhibitory concentration, and thus has the potential to be a promising treatment against gonorrhea. 

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Published

2020-04-20

Issue

Vol. 8 (2020)

Section

Articles