The Antibacterial Activity of ZrO2 Nanoparticles on Biocide Resistant Bacilli in Paints
DOI:
https://doi.org/10.12970/2311-1755.2014.02.02.2Keywords:
Antibacterial activity, Bacilli, Biocides, Biodeteriorated paints, Nanoparticles.Abstract
The antibacterial activity of four biocides against members of the class Bacilli isolated from paint and paint scrapings was investigated using the disc diffusion technique. Bacillus species were selected for this study amongst other organisms isolated from paint and paint scrapings because they were regularly isolated in highest populations when compared with other isolates detected. The biocides were made up of benzimidazole carbamate/2-n-octyl-4-isothiazolin -3-one (OIT)/Urea derivative), (5-Chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (CIT/MCIT); (Carbendazim octylisothiazolone and Diuron) and (Chloromethyl and methylisothiazolone). Bacillus cereus and B. coagulans had 0% inhibition of all the biocides tested. Similar experiments with ZrO2 nanoparticles yielded 65% inhibition of Bacillus sphaericus and 80% of Brevibacillus choshinensis. The result of this study indicates the need for more sophisticated antimicrobials and underscores the superior efficacy of nano-structured antimicrobials over conventional biocides used in the paint industry. These findings will enhance efforts geared towards combating biocide-resistant microorganisms in the paint industry.
References
Obidi OF, Aboaba OO, Makanjuola MS, Nwachukwu SCU. Microbial evaluation and deterioration of paints and paint-products. J Env Biol 2009; 30(5): 835-40.
Zaigham A, Basharat A, Ajum Naseem S. Antimicrobial activity of biocides against different microorganisms isolated from biodeteriorated paints. Pakistan J Zool 2012; 44(2): 576.
Dey BK, Hashim MA, Hassan S, Gupta BS. Microfilteration of water-based paint effluents. Adv Env Res 2004; 8(3): 455-66. http://dx.doi.org/10.1016/S1093-0191(02)00122-3
Morana A, Maurelli MA, Ionata E, La Cara F. Rossi M. Cellulases from fungi and bacteria and their biotechnological applications. In: Cellulase: types and action, mechanism and uses. Nanoscience Publishers 2011; pp. 1-80.
Taubel M, Kampfer P, Buczolits S, Lubitz W, Busse H. Bacillus barbaricus sp. Nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 2003; 53: pp. 725-30. http://dx.doi.org/10.1099/ijs.0.02304-0
Russell AD. Antibiotic and biocide resistance in bacteria: comments and conclusions”, J Appl Microbiol Symp 2002; 92(Suppl): 171S-173S. http://dx.doi.org/10.1046/j.1365-2672.92.5s1.11.x
Ito H, Ura A, Oyamada Y, et al. A 4-aminofurazan derivative-A 189-inhibits assembly of bacterial cell division protein FtsZ in vitro and in vivo. Microbiology and Immunol 2006; 50: 759-64. http://dx.doi.org/10.1111/j.1348-0421.2006.tb03851.x
Reedy KM, Feris K, Bell J, Wingett DG, Hanley C and Punnose A. Selective Toxicity of Zinc Oxide Nanoparticles to Prokaryotic and Eukaryotic Systems. Appl Phy Letts 2007; 90: 213902-3. http://dx.doi.org/10.1063/1.2742324
Azam A, Ahmed AS, Oves M, et al. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int J Nanoscience 2012; 2(7): 6003-9.
Mishra M, Paliwal JS, Singh SK, Selvarajan E, Subathradevi C, Mohanasrinivasan V. Studies on the inhibitory activity of biologically synthesized and characterized zinc oxide nanoparticles using Lactobacillus sporogens against Staphylococcus aureus. J Pure Appl Microbiol 2013; 7(2): 1-6.
Erdogan EE, Sahin F, Namli A. Phospholipid fatty acids analysis-fatty acid methyl ester (PLFA-FAME) changes during bioremediation of crude oil contamination soil. Afr J Biotech 2013; 12(44): 6294-301.
Joo J, Yu Y, Kim YM, et al. Multigram scale synthesis and characterization of monodisperse tetragonal zirconia nanocrstals. J Am Chem Soc 2003; 125(21): 6553-7. http://dx.doi.org/10.1021/ja034258b
Clinical and Laboratory Standards Institute (CLSI) Performance standards for antimicrobial disk susceptibility tests. Approved Standard - 9th ed., CLSI Document M2-A9. Wayne, PA: CLSI 2006.
Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. J Food Contrl 2010; 21: 1199-1218. http://dx.doi.org/10.1016/j.foodcont.2010.02.003
Gosh A, Das BK, Roy A, Mandal B, Chandra G. Antibacterial activity of some medicinal plant extracts. J Nat Med 2008; 62: 259-62. http://dx.doi.org/10.1007/s11418-007-0216-x
Lee SY, Oh HY, Kim OB. Isolation and Characterization of volatile organic compounds-degrading Bacillus strains from loess. J Environ Protect 2013; 4: 50-7. http://dx.doi.org/10.4236/jep.2013.44A007
Hatchett DW, Henry S. Electrochemistry of sulphur ad layers on the low-index faces of silver. J Phys Chem 1996; 100: 9854-9. http://dx.doi.org/10.1021/jp953757z
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 2004; 275: 177-82. http://dx.doi.org/10.1016/j.jcis.2004.02.012
Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium E. coli. Appl Environ Microbiol 2007; 73(6): 1712-20. http://dx.doi.org/10.1128/AEM.02218-06
Rupariela JP, Chatteerjee AK, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomaterialia 2008; 4: 707-17. http://dx.doi.org/10.1016/j.actbio.2007.11.006
Jangari SL, Stalin K, Dibaqui N, et al. Antimicrobial activity of zirconia (ZrO2) nanoparticles and zirconium complexes. J Nanosci Nanotechnol 2012; 12(9): 7105-12. http://dx.doi.org/10.1166/jnn.2012.6574
Mc Mullin RH. New aspects in the sustainability of nanoparticle-modified coatings. Nanotechnol NAFTA/BYK USA Inc. 2011; 30-4.
Sollazo V, Palmieri A, Pezzetti F, et al. Genetic effect of zirconium oxide coating on osteoblast-like cells. J Biomed Mater Res B Appl Biomater 2008; 84(2): 550-8. http://dx.doi.org/10.1002/jbm.b.30903
