Differences in Quality of DNA Isolated from Fresh and Frozen Peripheral Blood with Salting-Out Methods
DOI:
https://doi.org/10.12970/2311-1755.2019.07.02Keywords:
DNA Isolation, Salting-Out Methods, Fresh and Frozen Blood.Abstract
Today, a lot of genomics based research, including genomics, molecular biology, biotechnology and whole genome sequencing, is performed. DNA isolation using commercially available kits from peripheral blood is both costly and inefficient with respect to the amount of DNA isolated. In order to obtain high amounts of fine quality of DNA, the manual precipitation method by salting- out is more efficient. In this study, human fresh peripheral blood tissue and frozen blood tissue were used. DNA isolation from these two different types of blood samples was performed. The purity, quantity and PCR success of the DNA samples were evaluated. As a result, the concentration and purity of DNA isolated from fresh blood samples were determined by Nanodrop spectrophotometer and agarose gel analysis. The fresh blood samples yielded higher quantity, better purity and higher PCR compliance quality than frozen blood samples.
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