Bioactivity and Antibacterial Effects of Ag-Ca-P Doped PEO Titania Coatings

Authors

  • Luciane S. Santos Department of Mechanical Engineering, Polytechnic School, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil
  • Dhanna Francisco Department of Mechanical Engineering, Polytechnic School, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil
  • Evelyn Leite Dentistry Department, Universidade Estadual de Ponta Grossa, Ponta Grossa - PR, 84010-290 Brazil
  • Sheron Cogo Health Science Department, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil and Department of Mechanical Engineering, School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523 USA
  • Marcela Dias-Netipanyj Health Science Department, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil and Department of Mechanical Engineering, School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523 USA
  • Shelon Pinto Dentistry Department, Universidade Estadual de Ponta Grossa, Ponta Grossa - PR, 84010-290 Brazil
  • Selene Espósito Health Science Department, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil
  • Ketul Popat Department of Mechanical Engineering, School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523 USA
  • Paulo Soares Department of Mechanical Engineering, Polytechnic School, Pontifícia Universidade Católica do Paraná, Curitiba – PR 80215-901 Brazil

DOI:

https://doi.org/10.12970/2311-1755.2018.06.02

Keywords:

Plasma electrolytic oxidation, titanium, bactericidal coating, silver

Abstract

Implant centered infections remain as one of the main complications associated with the use of biomedical implants. These infections can be avoided with the development of bactericidal coatings that prevent bacterial contamination since the very early stage of implantation. However, a multifunctional coating should inhibit bacterial contamination without generating cytotoxic responses. To achieve this purpose, this work presents a comparative evaluation of coatings with different concentrations of Ag. Coatings containing silver, calcium and phosphorous were obtained by plasma electrolytic oxidation (PEO) and its bactericidal activity and cytotoxicity were evaluated against Staphylococcus aureus and adipose derived stem cells (ADSC), respectively. Silver, calcium and phosphorous were successfully incorporated in the coatings and silver has not affected the coating morphology nor the crystalline structure. ADSC viability was unaltered by cell growth over the surfaces, despite the observation of thinner cells on coatings with higher silver content. After 24 h of incubation, bactericidal activity was observed in coatings with more than 0.6 % at. Ag incorporated, while coatings with 0.2 % at. Ag presented an increased bacterial proliferation indicating a hormetic response. Thus, Ag-CaP-TiO2 coating could be a potential solution for the prevention of implant infections.

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Published

2018-04-11

Issue

Vol. 6 (2018)

Section

Articles