Tissue Engineering of Human Septal Cartilage Using a Rotary Bioreactor

Authors

  • Marsha S. Reuther Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA and Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, CA, USA
  • Van W. Wong Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA and Research Service, VA San Diego Healthcare System, San Diego, CA, USA
  • Kristen K. Briggs Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA and Research Service, VA San Diego Healthcare System, San Diego, CA, USA
  • Barbara L. Schumacher Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA and Research Service, VA San Diego Healthcare System, San Diego, CA, USA
  • Robert L. Sah Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
  • Koichi Masuda Department of Orthopedic Surgery, University of California, San Diego, La Jolla, CA, USA
  • Deborah Watson Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA and Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, CA, USA

DOI:

https://doi.org/10.12970/2311-1755.2014.02.01.3

Keywords:

 Cartilage tissue engineering, human nasal septal tissue, bioreactor, rotary vessel, cartilage constructs, cartilage reconstruction.

Abstract

Tissue engineering of human septal neocartilage offers the potential to produce large quantities of autologous material for use in the repair of cartilaginous craniofacial defects. Culture of septal neocartilage constructs in a rotary cell culture system bioreactor may improve the biochemical and biomechanical properties of engineered tissue. The objective of this study was to characterize the morphological, compositional and mechanical properties of engineered human septal cartilage constructs when cultured in three different environments. Human septal cartilage constructs were cultured in transwell plates for 6 weeks and subsequently cultured for an additional 4 weeks in transwell plates, free-floating in a sterile media bottle, or in a rotary cell culture system bioreactor. The histologic, biochemical, and biomechanical properties of the constructs were examined. All constructs maintained their radial dimensions throughout the culture period. Qualitatively, the constructs cultured in the rotary cell culture system bioreactor were firmer and more resilient compared with the constructs cultured under free-floating static and transwell plate conditions. Culture of human septal neocartilage constructs in a rotary cell culture system bioreactor augmented cell proliferation and extracellular matrix production when compared with constructs cultured in transwell plates and free-floating static conditions. Additionally, rotary cell culture system bioreactor culture enhanced total and type II collagen production. The rotary cell culture system bioreactor constructs possessed improved biomechanical properties compared with the other conditions and this is reflected in their superior resistance to manipulation. These findings suggest that culture in a rotary cell culture bioreactor can modulate the composition and mechanical function of engineered scaffold-free human septal cartilage constructs to produce constructs better suited for reconstructive surgery.

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Published

2014-04-05

Issue

Vol. 2 No. 1 (2014)

Section

Articles