SEFFI (Superficial Enhanced Fluid Fat Injection) for Aesthetic and Clinical Regenerative Treatments
Authors
Alessandro Gennai1, Silvia Zia2, Barbara Roda2,3, Alessia Maggio2, Laura Bonsi4, Francesco Alviano4, Andrea Zattoni2,3, Pierluigi Reschiglian2,3 and Francesco P. Bernardini5 1Plastic Reconstructive and Aesthetic Surgeon Medical Director STUDIO GENNAI, Bologna, Italy; 2Stem Sel srl, Bologna, Italy; 3Department of chemistry, University of Bologna, Italy; 4Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Italy; 5Oculoplasty Private practice in Genova, Italy
Liposuction cannula, fat graft, ASCs, regenerative medicine, cell production quality control system.
Abstract
Aim: In the field of regenerative applications, new fat preparations are an interesting perspective for easily available sources of highly potent staminal cells. In order to support the development of new robust techniques for regenerative purposes, stemness and proliferative properties of cellular content should be addressed. Methods: SEFFI is standardized technique and published by two of the Authors (AG, PFB). These technique aim to harvest and re-inject autologous microfragmented adipose tissue with minimum manipulation. In this study the harvesting and preparation procedure was performed by disposable kits SEFFILLER™ and SEFFICARE™ (Produced by SEFFILLINE srl Bologna Italy). These cannulas performed a selection of clusters dimension and therefore microfragmented adipose tissue were immediately plated in colture dishes to allow liberated cells to attach to plastic. Cellularity from both cannulas was calculate and adipose stem cells (ASCs) from SEFFILLER™ and SEFFICARE™ were characterized by proliferation assay and differentiation capacity towards mesenchymal lineages. Moreover, as a quality control system, a new technology named Celector® was used to identify cell heterogeneity and viability of expanded ASCs which can be used for further cell therapy approaches. Results: Microfragmented tissue, harvested by both cannulas, showed good number of adherent cells. Cells were vital and with an optimal proliferation ability. Celector® analysis confirmed the highly cell viability and sharing physical properties between ASCs from both cannulas. Isolated cells showed stemness characteristics due to their ability to differentiate towards adipogenic and chondrogenic lineages. Conclusion: These results confirmed the presence of regenerative elements in autologous graft of SEFFI tissue. With perspectives of applications in aesthetic and clinical field.
