Bioactivity and Antibacterial Effects of Ag-Ca-P Doped PEO Titania Coatings

Luciane S. Santos, Dhanna Francisco, Evelyn Leite, Sheron Cogo, Marcela Dias-Netipanyj, Shelon Pinto, Selene Espósito, Ketul Popat, Paulo Soares

Abstract


Implant centered infections remain as one of the main complications associated with the use of biomedical implants. These infections can be avoided with the development of bactericidal coatings that prevent bacterial contamination since the very early stage of implantation. However, a multifunctional coating should inhibit bacterial contamination without generating cytotoxic responses. To achieve this purpose, this work presents a comparative evaluation of coatings with different concentrations of Ag. Coatings containing silver, calcium and phosphorous were obtained by plasma electrolytic oxidation (PEO) and its bactericidal activity and cytotoxicity were evaluated against Staphylococcus aureus and adipose derived stem cells (ADSC), respectively. Silver, calcium and phosphorous were successfully incorporated in the coatings and silver has not affected the coating morphology nor the crystalline structure. ADSC viability was unaltered by cell growth over the surfaces, despite the observation of thinner cells on coatings with higher silver content. After 24 h of incubation, bactericidal activity was observed in coatings with more than 0.6 % at. Ag incorporated, while coatings with 0.2 % at. Ag presented an increased bacterial proliferation indicating a hormetic response. Thus, Ag-CaP-TiO2 coating could be a potential solution for the prevention of implant infections.

Keywords


Plasma electrolytic oxidation, titanium, bactericidal coating, silver.

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