Identification of miRNA-mRNA Interaction in Vero Cells after Infection of SADS-CoV through Integrative Transcriptome Analysis (Pages 19-27)

Yaoyao Zheng1,2, Jiaoling Wu1,2, Yuqi Chen1,2, Xiaoyu Tang1,2, Yaorong Tan1,2, Yuan Sun1,2, Qianniu Li1,2, Ruiting Wu1,2, Xiangbin Zhang1,2 and Jingyun Ma1,2

1College of Animal Science, South China Agricultural University, Guangzhou, China; 2Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China


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Abstract: Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus that can cause severe diarrhea in newborn piglets in China. Here, we reported profiles of differentially expressed miRNAs and mRNAs in Vero cells infected by SADS-CoV. In total, 345 miRNAs and 1014 mRNAs were identified with significantly differential expression after SADS-CoV infection. The analysis of miRNA-mRNA-pathway network indicated that through major mRNA nodes, TUBA, KRAB, eIF4E and SIRPA_B1_G, differentially expressed miRNAs were mainly involved in pathways of pathogenic Escherichia coli infection, apoptosis, PI3K-Akt signaling pathway, focal adhesion and so on. The qRT-PCR validation results of six selected genes were concordant with RNA-Seq results. This study is the first report to examine differentially expressed miRNAs and mRNAs after SADS-CoV infection in Vero cells, which may help elucidate the underlying mechanisms of SADS-CoV infection.

Keywords: SADS-CoV, Vero cells, Differentially expressed miRNA, Differentially expressed mRNA, miRNA-mRNA-pathway network.
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