Effect of Conjugated Linoleic Acid (CLA) in Rats Subjected to Damage Liver Induced by Carbon Tetrachloride Pages 32-42
Eliane Bonifácio Teixeira de Carvalho1, Illana Louise Pereira de Melo1, Ana Mara de Oliveira e Silva2, Dalva Assunção Portari Mancini3 and Jorge Mancini-Filho1
1Laboratory of Lipids, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo; 2Center for Nutrition, University of Sergipe; 3Butantan Institute, São Paulo, Brazil
Abstract: The conjugated linoleic acid (CLA) consists in a group of positional and geometrical isomers of octadecadienoic acid (18:2 ω-6) in which the two double bonds are conjugated and may have cis or trans configurations. CLAs have been the subject of extensive investigations for their various biological activities, including antiatherosclerotic, anticarcinogenic and antioxidative activities. The aim of this study was to evaluate the antioxidant activity and hepatoprotective properties of CLA in rats subjected to liver injury induced by carbon tetrachloride (CCl4). Eighteen male Wistar rats were divided into three groups: control; CCl4 and CLA. The CLA was administrated daily by gavage for 21 days, and then subjected to liver injury induced by CCl4. The serum and liver were collected and analyzed. CLAs supplementation take to attenuation of the liver damage in treated animals, since the reduction in the levels of thiobarbituric acid reactive substances (TBARS) in the liver suggests hepatoprotective activity of these fatty acids. The levels of catalase (CAT) and glutathione reductase (GR) and reduced glutathione (GSH) levels in liver tissue as a result of reduced oxidative damage induced by CCl4, and increased after treatment with CLA, suggesting antioxidant capacity of CLAs. Moreover the analysis of reverse transcription / polymerase chain reaction (RT / PCR) showed increased expression of the CAT gene, suggesting inducing effects on this enzyme. These results suggest that CLAs can be used as adjuncts to attenuate hepatic damage.
Keywords: Conjugated linoleic acid, hepatoprotective, carbon tetrachloride, antioxidant activity, rats. Read more